| Catalog#: |
MK0588H |
| Product Name: |
Recombinant Human PCNA, His tagged |
| Product Overview: |
Recombinant Human PCNA (P12004) mature form (Met 1-Ser 261), fused with a polyhistidine tag at the N-terminus, was produced in Baculovirus-Insect cells. |
| Description: |
The DNA PCNA is central to both DNA replication and repair. Two forms of PCNA exist in cells: a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and a soluble form in quiescent cells in G1 and G2 phases. PCNA is an essential protein for replication of chromosomal DNA. |
| Source: |
Baculovirus-Insect cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from 0.2μm filtered solution of 50mM Na3PO4, 300mM NaCl, 10% glycerol, pH 7.0, 2mM DTT |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31 kDa. It migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
