| Catalog#: |
MK0555H-B |
| Product Name: |
Recombinant Human Met protein, His-Fc-tagged, Biotinylated |
| Product Overview: |
Recombinant Human Met protein extracellular domain (NP_000236)(Met 1-Thr 932), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was expressed in HEK293 cells Cells. The purified protein was biotinylated in vitro. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
Fc-His |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80. |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
