| Catalog#: |
MK0977H |
| Product Name: |
Recombinant Human LRRK2 (I2020T), GST-tagged, Active |
| Product Overview: |
Recombinant human LRRK2 (I2020T) (968-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description: |
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. Mutations in LRRK2 are the most frequent known cause of autosomal dominant and idiopathic Parkinson"s disease with prevalent mutations being found within the GTPase and kinase domains. LRRK2 cooperates with MET to promote efficient tumor cell growth and survival in various cancers. Down-regulation of LRRK2 in cultured tumor cells compromises MET activation and selectively reduces downstream MET signaling to mTOR and STAT3. |
| Source: |
Sf9 insect cells |
| Species: |
Human |
| Tag: |
GST |
| Formulation: |
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Bio-activity: |
The specific activity of LRRK2 was determined to be 7 nmol /min/mg |
| Purity: |
>70% by densitometry |
| Storage: |
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Molecular Mass: |
~210 kDa |
