| Catalog#: |
MK0927H |
| Product Name: |
Recombinant Human LGR5 Protein, Fc Chimera |
| Product Overview: |
Recombinant Human LGR5 (Accession # O75473), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived. |
| Source: |
CHO |
| Species: |
Human |
| Tag: |
Fc |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS. |
| Bio-activity: |
Measured by its binding ability in a functional ELISA.When Recombinant Human R-Spondin 3 is coated at 0.5 µg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd <0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 and 5 ng/mL Recombinant Mouse Wnt-3a. |
| Purity: |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage: |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
| Molecular Mass: |
Recombinant Human LGR5, Fc Chimera has a calculated MW of 87 kDa (monomer). In SDS-PAGE migrates as 90-110 kDa, reducing conditions. |
