| Catalog#: |
MK0520H |
| Product Name: |
Recombinant Human KIT Protein (Thr540-Val972), His-GST-tagged |
| Product Overview: |
Recombinant Human KIT Protein isoform 2 (P10721-2) cytoplasmic domain (Thr540-Val972) was produced by Baculovirus-Insect cells expression system. This protein was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Source: |
Baculovirus-Insect cells |
| Species: |
Human |
| Tag: |
His&GST |
| Formulation: |
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 3mM DTT, 5%~8% trehalose and mannitol, 0.01% Tween80. |
| Protein length: |
Thr540-Val972 |
| Purity: |
>92% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human KIT(aa 540-972) /GST chimera consists of 670 amino acids and predictes a molecular mass of 76.8 kDa. It migrates as an approximately 68 KDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
