| Catalog#: |
MK0499H |
| Product Name: |
Recombinant Human KDR protein, His-tagged |
| Product Overview: |
Recombinant Human KDR (P35968) aa. (Ala20~Glu764) fused with His tag was produced in HEK293 cellsF cells. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300. |
| Protein length: |
Ala20~Glu764 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. Do not vortex. |
| Applications: |
SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling |
| Molecular Mass: |
Predicted Molecular Mass: 84.9kDa; Accurate Molecular Mass: 90kDa as determined by SDS-PAGE reducing conditions. |
| Stability: |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
