| Catalog#: |
MK0502H |
| Product Name: |
Recombinant Human KDR, His-tagged, Biotinylated |
| Product Overview: |
The recombinant human KDR/VEGFR2/CD309 extracellular or ecto-domain (ECD) is expressed as a 756 amino acid protein consisting of Ala20 - Glu764 region of KDR and a C-terminal His-tag. It contains 18 potential N-linked glycosylation sites. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule). |
| Protein length: |
Ala20 - Glu764 |
| AA Sequence: |
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVI GNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEK RFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKL VLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGITRSDQGLYTCAASSGLMTK KNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSE RDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEE CANEPSHAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRG ERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLN ATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVS CTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKT NLESTGHHHHHHHH |
| Purity: |
>90% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Molecular Mass: |
Calculated molecular mass (kDa): 84.6; Estimated by SDS-PAGE under reducing condition (kDa): 110-120 suggesting that the protein is glycosylated. |
| Endotoxin: |
Less than 0.1 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
