| Catalog#: |
MK0444H |
| Product Name: |
Recombinant Human IGF1R, His & GST tagged |
| Product Overview: |
Recombinant Human IGF1R (NP_000866.1) cytoplasmic domain (Met 954-Cys 1367), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells. |
| Description: |
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. |
| Source: |
Baculovirus-Insect cells |
| Species: |
Human |
| Tag: |
His&GST |
| Formulation: |
Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 20% glycerol, 0.3mM DTT |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
