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Recombinant Human IDH1 Protein, His-tagged

Product Information
Catalog#:  MK0931H
Product Name:  Recombinant Human IDH1 Protein, His-tagged
Product Overview:  Recombinant C-terminal His-tagged human IDH1 protein was expressed in E. coli.
Description:  Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Source:  E. coli
Species:  Human
Tag:  His
Formulation:  50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM β-mercaptoethanol, 50 mM calcium chloride, and 20% glycerol
Purity:  ≥90% as determined by SDS-PAGE
Storage:  At -80 centigrade.
Molecular Mass:  47.9 kDa
Stability:  ≥ 6 months
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