| Product Name: |
Recombinant Human HGFR/c-MET Protein, His-Avi tagged, Biotinylated |
| Catalog#: |
MK1038H |
| Species: |
Human |
| Source: |
HEK293 |
| Tag: |
His-Avi |
| Product Overview: |
Biotinylated recombinant Human HGFR/c-MET Protein (P08581-1) (Glu25-Thr932), fused with a C-terminal His-Avi tag, was expressed in HEK293 cells. |
| Description: |
HGFR (Hepatocyte Growth Factor Receptor) is a receptor tyrosine kinase that plays a pivotal role in epithelial morphogenesis and cancer development. The mature, active form of this glycosylated receptor consists of an alpha and a beta subunit, which are derived from a single-chain precursor through post-translational cleavage and then joined by a disulfide bond. The extracellular domain of human HGFR is highly conserved across mammalian species, sharing 86-88% amino acid sequence identity with its canine, mouse, and rat counterparts, highlighting its fundamental biological importance. |
| Formulation: |
Formulated in 0.22μm-filtered Phosphate-Buffered Saline (pH 7.4). |
| Purity: |
> 95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
Use distilled water to dissolve the protein to a concentration above 100 μg/mL. Aliquot the solution into single-use vials and freeze. |
| Molecular Mass: |
The mature HGF Receptor (HGFR) is a heterodimer composed of a disulfide-linked alpha (α) and beta (β) chain. While the predicted molecular weights of the α and β chains are 32.5 kDa and 72.1 kDa respectively, the protein exhibits a higher apparent molecular weight due to post-translational glycosylation. As a result, when analyzed by Tris-Bis PAGE, the glycosylated α-chain migrates at approximately 45-50 kDa and the β-chain migrates at 80-90 kDa. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures. Aoid repeated freeze-thaw cycles. |
