| Catalog#: |
MK0388H |
| Product Name: |
Recombinant Human FLT4 protein, His & Avi-tagged, Biotinylated |
| Product Overview: |
Biotinylated Human VEGF R3, His Tag is expressed from human 293 cells (HEK293 cells). It contains AA Tyr 25 - Ile 776 (Accession # P35916-1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His&Avi |
| Formulation: |
Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4, 10% trehalose. |
| Protein length: |
Tyr 25 - Ile 776 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 88.2 kDa. The protein migrates as 115-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
