| Catalog#: |
MK0342H |
| Product Name: |
Recombinant Human ERBB3, His-tagged, Biotinylated |
| Product Overview: |
Human ErbB-3 /HER3 Protein, Biotinylated (Biotinylated rhErbB3) Ser 20 - Thr 643, was labeled with biotin based on rhErbB3 Protein, which was produced in human 293 cells (HEK293 cells). |
| Description: |
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or diffe |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Purity: |
>95% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
Biotinylated rhErbB3, fused with 6×His tag at the C-terminus, has a calculated MW of 71.5 kDa. The predicted N-terminus is Ser 20. DTT-reduced Protein migrates as 100-110 kDa due to glycosylation. |
| Stability: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
