| Catalog#: |
MK0284H |
| Product Name: |
Recombinant Human EPCAM Protein, His & Avi-tagged, Biotinylated |
| Product Overview: |
Recombinant Human EPCAM Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293 cells). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). Predicted N-terminus: Gln 24. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 30 kDa. The protein migrates as 36-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His&Avi |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Protein length: |
Gln 24 - Lys 265 |
| Purity: |
>95% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
30 kDa |
| Stability: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
