| Catalog#: |
MK0282H |
| Product Name: |
Recombinant Human EPCAM, His-tagged, Biotinylated |
| Product Overview: |
Human EpCAM /CD326 Protein, Biotinylated (Biotinylated EpCAM) Gln 24 - Lys 265, was labeled with biotin based on rhEpCAM Protein, which was produced in human 293 cells (HEK293 cells). |
| Description: |
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Purity: |
>95% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
Biotinylated EpCAM, fused with 6×His tag at the C-terminus, has a calculated MW of 29 kDa. The predicted N-terminus is Gln 24. DTT-reduced Protein migrates as 33-36 kDa due to glycosylation. |
| Stability: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
