| Catalog#: |
MK0258H |
| Product Name: |
Recombinant Human EGFRvIII Protein, His-tagged |
| Product Overview: |
Recombinant Human EGFRvIII Protein, His-tagged is expressed from human HEK293 cells cells. It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Protein length: |
Leu 25 - Ser 645 |
| Purity: |
>95% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
40.5 kDa |
| Stability: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
