| Catalog#: |
MK0254H |
| Product Name: |
Recombinant Human EGFR Protein, His-Avi-tagged, Biotinylated |
| Product Overview: |
Biotinylated Human EGFRvIII is expressed from HEK293 cells cells. It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His&Avi |
| Formulation: |
Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4, 10% trehalose. |
| Protein length: |
Leu 25 - Ser 645 |
| Purity: |
>95% as determined by reducing SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing condition, and 60-70 kDa under non-reducing condition (SDS-PAGE). |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C, three months at -20°C to -80°C after reconstitution. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
