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Recombinant Human CTSB protein, His-tagged

Product Information
Catalog#:  MK0216H
Product Name:  Recombinant Human CTSB protein, His-tagged
Product Overview:  Recombinant Human CTSB protein (Arg18-Ile339), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293 cells).
Source:  HEK293 cells
Species:  Human
Tag:  His
Formulation:  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose.
Protein length:  322
Purity:  >95% as determined by SDS-PAGE.
Storage:  Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Reconstitution:  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.
Molecular Mass:  The protein has a calculated MW of 36.7 kDa (pro-form) and 29.0 kDa (mature-form). Due to glycosylation, The reducing (R) protein migrates as 42-50 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
Endotoxin:  Less than 1.0 EU per μg as determined by the LAL method.
Notes:  Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles.
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