| Product Information | |
|---|---|
| Catalog#: | MK0216H |
| Product Name: | Recombinant Human CTSB protein, His-tagged |
| Product Overview: | Recombinant Human CTSB protein (Arg18-Ile339), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293 cells). |
| Source: | HEK293 cells |
| Species: | Human |
| Tag: | His |
| Formulation: | Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose. |
| Protein length: | 322 |
| Purity: | >95% as determined by SDS-PAGE. |
| Storage: | Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: | The protein has a calculated MW of 36.7 kDa (pro-form) and 29.0 kDa (mature-form). Due to glycosylation, The reducing (R) protein migrates as 42-50 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band. |
| Endotoxin: | Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: | Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
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