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Recombinant Human CD73/NT5E Protein, His&Avi-tagged, Biotinylated

Product Information
Catalog#:  MK1211H
Product Name:  Recombinant Human CD73/NT5E Protein, His&Avi-tagged, Biotinylated
Description:  CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. The enzyme consists of a dimer of 2 identical 70 kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Source:  HEK293 cells
Species:  Human
Tag:  His&Avi
Formulation:  Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5).
Protein length:  Trp27-Cys554
Purity:  > 95% as determined by Bis­Tris PAGE.
Storage:  Store under sterile conditions at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles.
Reconstitution:  Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Molecular Mass:  60.92 kDa
Stability:  Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C.
Endotoxin:  Less than 1 EU per μg by the LAL method.
Notes:  Research Use Only. Not for use in clinical procedures.
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