| Catalog#: |
MK1190H |
| Product Name: |
Recombinant Human CD3E (Asp23-Asp126) & CD3D (Phe22-Ala105) Protein, His-tagged |
| Description: |
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Protein length: |
Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D) |
| Purity: |
> 95% as determined by Bis-Tris PAGE.
> 95% as determined by HPLC. |
| Storage: |
Store under sterile conditions at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
| Molecular Mass: |
17.9 kDa (CD3E) and 16.7 kDa (CD3D) |
| Stability: |
Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Recommend to aliquot the protein into smaller quantities for optimal storage. |
| Endotoxin: |
Less than 1 EU per μg by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures. |
