| Catalog#: |
MK0929H |
| Product Name: |
Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin |
| Product Overview: |
Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1). Predicted N-terminus: Phe 19. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Source: |
HEK293 |
| Species: |
Human |
| Tag: |
LIgG2b Fc |
| Formulation: |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity: |
Immobilized Human PD-1, His Tag at 1 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.078-2.5 μg/mL (QC tested).
Immobilized Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc Tag with a linear range of 20-313 ng/mL (Routinely tested). |
| Protein length: |
Phe 19- Arg 238 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Molecular Mass: |
53.0 kDa |
| Stability: |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Endotoxin: |
Less than 0.01 EU per μg by the LAL method. |
