| Catalog#: |
MK0930H |
| Product Name: |
Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated |
| Product Overview: |
Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Source: |
HEK293 |
| Species: |
Human |
| Tag: |
His & Fc & Avi |
| Formulation: |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity: |
Immobilized Human PD-1, Fc Tag at 2 μg/mL (100μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.01-0.25 μg/mL (QC tested).
Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2μg/ml. The IC50 is 0.64 μg/ml (Routinely tested).
Immobilized cell surface PD-1(5x104 of cells per well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with an EC50 of 0.082ug/ml (Routinely tested). |
| Protein length: |
Phe 19 - Arg 238 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Molecular Mass: |
53.2 kDa |
| Stability: |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Endotoxin: |
Less than 1.0 EU per μg by the LAL method. |
