| Catalog#: |
MK0936H |
| Product Name: |
Recombinant Human CD19 protein, Fc/Avi-tagged, Biotinylated |
| Product Overview: |
Biotinylated Recombinant Human CD19(Glu21- Lys291) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
| Description: |
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). Mature human CD19 consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19. CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). |
| Source: |
CHO |
| Species: |
Human |
| Tag: |
Fc/Avi |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity: |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Protein length: |
Glu21- Lys291 |
| Purity: |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution: |
Reconstitute at 500 μg/mL in PBS. |
| Molecular Mass: |
75-95 kDa, under reducing conditions |
| Endotoxin: |
<0.10 EU per 1 μg of the protein by the LAL method. |
