| Catalog#: |
MK0065H |
| Product Name: |
Recombinant Human CASP3, His tagged |
| Product Overview: |
Recombinant Human CASP3 mature form (Q5IS54) (Ser29-His277), fused with a polyhistidine tag at the C-terminus, was produced in E. coli. |
| Description: |
Caspase-3, also known as CASP-3, is a member of the cysteine-aspartic acid protease (caspase) family. It is highly expressed in lung, spleen, heart, liver and kidney. Caspase-3 also can be detected in many cell lines. Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase-3 also cleaves and activates sterol regulatory element binding protein |
| Source: |
E. coli |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5, 5%~8% trehalose and mannitol. |
| Purity: |
(48.4+48.1)% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
