| Catalog#: |
MK1155H |
| Product Name: |
Recombinant Human B7-H3 Protein, His-tagged |
| Description: |
Human B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptor. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to co-stimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-γ production. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
His |
| Protein length: |
Leu29-Pro245 |
| Purity: |
> 95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/mL. |
| Molecular Mass: |
40-42 kDa, on SDS-PAGE under reducing conditions. |
| Stability: |
Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. |
| Endotoxin: |
< 0.2 EU/μg of protein by gel clotting method. |
| Notes: |
Research Use Only. Not for use in clinical procedures. |
