| Catalog#: |
MK0036H |
| Product Name: |
Recombinant Human APEX1, His tagged |
| Product Overview: |
Recombinant Human APEX1 (P27695) (Pro2-Leu 318), fused with a polyhistidine tag at the N-terminus, was produced in E. coli. |
| Description: |
APEX nuclease is a mammalian DNA repair enzyme having apurinic/apyrimidinic endonuclease, 3-5-exonuclease, DNA 3 repair diesterase and DNA 3-phosphatase activities. It is also a redox factor (Ref-1), stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun.In the embryonic and postnatal development, APEX mRNA was expressed at high levels in the proliferative zone of various brain regions, with showing temporal and spatial changes. |
| Source: |
E. coli |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.5. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human APEX1 consisting of 328 amino acids and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
