| Catalog#: |
MK0953H |
| Product Name: |
Recombinant Human AMH Protein (Leu25-Arg560), His-tagged |
| Product Overview: |
Recombinant Human AMH Protein (NP_000470.2) (Leu25-Arg560) was produced by CHO Cells expression system. This protein was expressed with a polyhistidine tag at the N-terminus. |
| Description: |
Anti-Mullerian hormone (AMH), a member of the TGF-beta superfamily, is produced by granulosa cells (GCs) of preantral and small antral follicles and plays a role in regulating the recruitment of primordial follicles and the FSH-dependent development of follicles. BMP15 up-regulates the transcription of AMH and that the inhibition of p38 MAPK decreases the BMP15-induced expression of AMH and SOX9, suggesting that BMP15 up-regulates the expression of AMH via the p38 MAPK signaling pathway, and this process involves the SOX9 transcription factor. AMH is widely used for assessing ovarian reserve, and it is particularly convenient, because it is thought to have minimal variability throughout the menstrual cycle. Fetal anti-Mullerian hormone (AMH) is responsible for normal male sexual differentiation, and circulating AMH is used as a marker of testicular tissue in newborns with disorders of sex development. Anti-Mullerian hormone (AMH) produced in the developing testis induces the regression of the Mullerian duct, which develops into the oviducts, uterus and upper vagina. As well as other hormone receptors, and a decreased ovarian cortex cell proliferation. These results help understanding the inhibitory effects of AMH on follicular development. |
| Source: |
CHO |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Protein length: |
Leu25-Arg560 |
| Purity: |
> 95% as determined by SDS-PAGE. |
| Storage: |
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human AMH consists of 555 amino acids and predicts a molecular mass of 59.1 kDa. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70 centigrade. |
| Endotoxin: |
< 1.0 EU per μg of the protein as determined by the LAL method. |
